ABSTRACT
Objective: To develop an HPLC-ELSD method for the simultaneous determination of six saponins constituents including notoginsenoside R1, ginsenoside Rg1, ginsenoside Re, ginsenoside Rb1, ginsenoside Rc and ginsenoside Rd in Xinling pills.Methods: HPLC-ELSD was used, and the chromatographic separation was performed on an Agilent Eclipse XBD-C18 column (150 mm×4.6 mm,5 μm) with acetonitrile-water as the mobile phase with gradient elution at the flow rate of 1.0 ml·min-1, the column temperature was maintained at 20℃, the drift tube temperature was 60℃, and the gas pressure was 4.00 bar.Results: Notoginsenoside R1, ginsenoside Rg1, ginsenoside Re, ginsenoside Rb1, ginsenoside Rc and ginsenoside Rd was linear within the range of 0.30-6.00μg(r=0.999 5), 1.14-22.80μg (r=0.999 6), 0.17-3.40 μg (r=0.999 7), 0.81-16.20 μg (r=0.999 7), 0.08-1.60 μg (r=0.999 8) and 0.07-1.40 μg (r=0.999 8), respectively.The average recovery was 98.23%, 97.98%, 99.14%, 99.15%, 98.72% and 98.37%, and the RSDs were 1.56%, 1.31%, 1.16%, 1.07%, 0.73% and 0.92%(n=6), respectively.Conclusion: The method is convenient, accurate and reproducible in the quality control of saponins components in Xinling pills
ABSTRACT
Objective To investigate the status of self-controlling blood pressure and their hospitalization reasons in primary hypertensive inpatients. Method A cross-sectional survey was performed in 206 patients with primary hypertension in the hospital. Results The 58 patients did not know that they previously suffered from hypertension. They had to be in the hospital as stroke or cardiovascular disease. Those who had measures of self-controlling blood pressure were taken a proportion of 59.22%. The proportion of those who regularly took drugs was only 10.68%. The regular checking rate of blood pressures was 12.14%. Because there was not any or irregular measures, it was increased that the hypertensive patients with stroke or cardiovascular disease in the hospital. Conclusion It is one of the major factors for increasing patients with hypertension in the hospital that lacking the know-ledge which is relevant to prevention and treatment of hypertension. The results indicated that medical staff on non-communicative diseases prevention should educate the hypertensive patients, enhance self-protecting awareness and encourage them to cultivate health lifestyles and simultaneously take comprehensive prevention and treatment measure on hypertension. These suggest it be also necessary to carry out health education in border town especially in rural area and minority nationality regions.